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antihuman cd31 pecam 1 dylight 550  (Novus Biologicals)


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    Novus Biologicals antihuman cd31 pecam 1 dylight 550
    Antihuman Cd31 Pecam 1 Dylight 550, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd31+pecam+1/CD31%2FPECAM-1+Antibody+(C31%2E3+%2B+JC%2F70A)+%5BDyLight+550%5D/pm42826194-367-36-43
    Average 94 stars, based on 1 article reviews
    antihuman cd31 pecam 1 dylight 550 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Cellular and exosomal GPx1 are essential for controlling hydrogen peroxide balance and alleviating oxidative stress in hypoxic glioblastoma
    Article Snippet: For human specimen staining studies, frozen sections of human glioblastoma were incubated with primary antibodies, GPx1 (1:200 dilution; Thermo Fisher Scientific) with or without HIF-1α (1:150 dilution; Novus Biologicals), overnight at 4 °C and secondary antibodies, DyLght 649-conjugated goat anti-rabbit antibody (1:100 dilution; Molecular Probes) or Dylight 488-conjugated goat anti-mouse antibody (1:100 dilution; Abcam). .. To stain for CD31/PECAM-1, the tumor sections were incubated with primary antibodies, CD31/PECAM-1(1:100 dilution; Novus Biologicals), overnight at 4 °C and secondary antibodies, DyLght 649-conjugated goat anti-rabbit antibody (1:100 dilution; Molecular Probes). .. TUNEL staining was carried out using the TUNEL Apoptosis Detection Kit (Millipore) according to the manufacturer's protocol.

    Article Title: Cellular and exosomal GPx1 are essential for controlling hydrogen peroxide balance and alleviating oxidative stress in hypoxic glioblastoma.
    Article Snippet: For human specimen staining studies, frozen sections of human glioblastoma were incubated with primary antibodies, GPx1 (1:200 dilution; Thermo Fisher Scientific) with or without HIF-1α (1:150 dilution; Novus Biologicals), overnight at 4 ◦C and secondary antibodies, DyLght 649-conjugated goat anti-rabbit antibody (1:100 dilution; Molecular Probes) or Dylight 488-conjugated goat antimouse antibody (1:100 dilution; Abcam). .. To stain for CD31/PECAM-1, the tumor sections were incubated with primary antibodies, CD31/ PECAM-1(1:100 dilution; Novus Biologicals), overnight at 4 ◦C and secondary antibodies, DyLght 649-conjugated goat anti-rabbit antibody (1:100 dilution; Molecular Probes). .. TUNEL staining was carried out using the TUNEL Apoptosis Detection Kit (Millipore) according to the manufacturer’s protocol.

    Article Title: Bioprinting of Perfusable, Biocompatible Vessel-like Channels with dECM-Based Bioinks and Living Cells
    Article Snippet: Immunohistochemical staining was performed using ImmPRESS Duet Double Staining Polymer Kit (Vector, Newark, CA, USA; cat. no.: MP-7724) according to the manufacturer’s instruction. .. Briefly, for e-cadherin or CD31/PECAM-1 protein staining, following the standard microwave heat-induced unmasking procedure in citrate buffer (10 mM citric acid, 0.05% Tween 20, pH 6.0), slices were incubated in BLOXALL Blocking Solution (Vector, ImmPRESS Duet, MP-7724) for 10 min. After blocking in 10% normal horse serum for 1 h at room temperature, the sections were incubated with e-cadherin (Santa Cruz, Dallas, TX, USA, cat. no.: sc-8426) or CD31/PECAM-1 (Novus Biological, Centennial, CO, USA; cat. no.:NB100-2284) primary antibody overnight at 4 °C. ..

    Incubation:

    Article Title: Cellular and exosomal GPx1 are essential for controlling hydrogen peroxide balance and alleviating oxidative stress in hypoxic glioblastoma
    Article Snippet: For human specimen staining studies, frozen sections of human glioblastoma were incubated with primary antibodies, GPx1 (1:200 dilution; Thermo Fisher Scientific) with or without HIF-1α (1:150 dilution; Novus Biologicals), overnight at 4 °C and secondary antibodies, DyLght 649-conjugated goat anti-rabbit antibody (1:100 dilution; Molecular Probes) or Dylight 488-conjugated goat anti-mouse antibody (1:100 dilution; Abcam). .. To stain for CD31/PECAM-1, the tumor sections were incubated with primary antibodies, CD31/PECAM-1(1:100 dilution; Novus Biologicals), overnight at 4 °C and secondary antibodies, DyLght 649-conjugated goat anti-rabbit antibody (1:100 dilution; Molecular Probes). .. TUNEL staining was carried out using the TUNEL Apoptosis Detection Kit (Millipore) according to the manufacturer's protocol.

    Article Title: Sympathetic innervation of inguinal white adipose tissue in the mouse
    Article Snippet: Samples were incubated in permeabilization solution (400 ml PTx.2, 11.5 g glycine, 100 ml dimethylsylfoxid (DMSO)), followed by incubation in blocking solution (42 ml PTx.2, 5 ml DMSO, 3 ml normal donkey serum (NDS)) at 37°C for 2 days in a shaking incubator (Corning LSE Product #6790, Corning, NY). .. Then, tissues were incubated with primary antibodies (chicken anti‐GFP [1:400], Abcam, Cambridge, MA; rabbit anti‐TH [1:400], Millipore, Burlington, MA; CD31/PECAM‐1 [1:300], Novus Biologicals, Littleton, CO) in primary incubation solution (92 ml PTwH (1 ml of 10 mg/ml heparin stock solution, 2ml Tween‐20, quantum satis to 1 liter with PBS), 5 ml DMSO, 3 ml NDS) on a shaking incubator at 37°C for at least 7 days. .. Following primary staining, tissues underwent five, 60‐min washes in PTwH then subjected to secondary staining (AlexaFluor 647 donkey anti‐chicken [1:300], AlexaFluor 555 donkey anti‐rabbit [1:300], Jackson ImmunoResearch, West Grove, PA, AlexaFluor 488 donkey anti‐goat [1:300], ThermoFisher Scientific, Waltham, MA) in secondary incubation solution (PTwH with 3% NDS) for at least 7 days at 37°C on a shaking incubator.

    Article Title: Evaluating Ocular Response in the Retina and Optic Nerve Head after Single and Fractionated High-Energy Protons
    Article Snippet: Six μm sections were deparaffinized in Histoclear, rehydrated and, washed in PBS for 20 min. Vasculature was labeled with DyLight ® 488 Lycopersicon Esculentum (Tomato) Lectin (Vector Laboratories) for 30 min at room temperature followed by 10 min wash in PBS. .. Sections were then incubated overnight at 4 °C with primary rabbit antibodies against ZO-1 (Thermo Fisher Scientific, Hampton, NH, USA) or CD31/PECAM-1 (Novus Biologicals, Centennial, CO, USA) in antibody dilution buffer. .. After 3 washes in PBS, sections were incubated for 1.5 h with secondary antibody goat anti-rabbit IgG Alexa Fluor ® 568 (Life Technologies) followed by PBS washes.

    Article Title: Cellular and exosomal GPx1 are essential for controlling hydrogen peroxide balance and alleviating oxidative stress in hypoxic glioblastoma.
    Article Snippet: For human specimen staining studies, frozen sections of human glioblastoma were incubated with primary antibodies, GPx1 (1:200 dilution; Thermo Fisher Scientific) with or without HIF-1α (1:150 dilution; Novus Biologicals), overnight at 4 ◦C and secondary antibodies, DyLght 649-conjugated goat anti-rabbit antibody (1:100 dilution; Molecular Probes) or Dylight 488-conjugated goat antimouse antibody (1:100 dilution; Abcam). .. To stain for CD31/PECAM-1, the tumor sections were incubated with primary antibodies, CD31/ PECAM-1(1:100 dilution; Novus Biologicals), overnight at 4 ◦C and secondary antibodies, DyLght 649-conjugated goat anti-rabbit antibody (1:100 dilution; Molecular Probes). .. TUNEL staining was carried out using the TUNEL Apoptosis Detection Kit (Millipore) according to the manufacturer’s protocol.

    Article Title: Bioprinting of Perfusable, Biocompatible Vessel-like Channels with dECM-Based Bioinks and Living Cells
    Article Snippet: Immunohistochemical staining was performed using ImmPRESS Duet Double Staining Polymer Kit (Vector, Newark, CA, USA; cat. no.: MP-7724) according to the manufacturer’s instruction. .. Briefly, for e-cadherin or CD31/PECAM-1 protein staining, following the standard microwave heat-induced unmasking procedure in citrate buffer (10 mM citric acid, 0.05% Tween 20, pH 6.0), slices were incubated in BLOXALL Blocking Solution (Vector, ImmPRESS Duet, MP-7724) for 10 min. After blocking in 10% normal horse serum for 1 h at room temperature, the sections were incubated with e-cadherin (Santa Cruz, Dallas, TX, USA, cat. no.: sc-8426) or CD31/PECAM-1 (Novus Biological, Centennial, CO, USA; cat. no.:NB100-2284) primary antibody overnight at 4 °C. ..

    Double Immunostaining:

    Article Title: Lymphatic dysfunction in lupus contributes to cutaneous photosensitivity and lymph node B cell responses
    Article Snippet: .. Double immunostaining was performed with 1:40 dilution of D2-40, lymphatic endothelial cell marker mouse monoclonal antibody (Cat# 916602, Biolegend) and 1:100 dilution of CD31/PECAM-1, endothelial cell marker rabbit polyclonal antibody (#NB100-2284, Novus Biologicals) overnight at 4°C. ..

    Marker:

    Article Title: Lymphatic dysfunction in lupus contributes to cutaneous photosensitivity and lymph node B cell responses
    Article Snippet: .. Double immunostaining was performed with 1:40 dilution of D2-40, lymphatic endothelial cell marker mouse monoclonal antibody (Cat# 916602, Biolegend) and 1:100 dilution of CD31/PECAM-1, endothelial cell marker rabbit polyclonal antibody (#NB100-2284, Novus Biologicals) overnight at 4°C. ..

    Blocking Assay:

    Article Title: Bioprinting of Perfusable, Biocompatible Vessel-like Channels with dECM-Based Bioinks and Living Cells
    Article Snippet: Immunohistochemical staining was performed using ImmPRESS Duet Double Staining Polymer Kit (Vector, Newark, CA, USA; cat. no.: MP-7724) according to the manufacturer’s instruction. .. Briefly, for e-cadherin or CD31/PECAM-1 protein staining, following the standard microwave heat-induced unmasking procedure in citrate buffer (10 mM citric acid, 0.05% Tween 20, pH 6.0), slices were incubated in BLOXALL Blocking Solution (Vector, ImmPRESS Duet, MP-7724) for 10 min. After blocking in 10% normal horse serum for 1 h at room temperature, the sections were incubated with e-cadherin (Santa Cruz, Dallas, TX, USA, cat. no.: sc-8426) or CD31/PECAM-1 (Novus Biological, Centennial, CO, USA; cat. no.:NB100-2284) primary antibody overnight at 4 °C. ..



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